This position is grant funded

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

LAB RESEARCH ANALYST I

Yeh Lab – Duke Human Vaccine Institute

 

Overview

The Duke Human Vaccine Institute (DHVI) is at the forefront of groundbreaking vaccine research targeting infectious diseases with significant global health impacts. Through collaborative efforts, DHVI teams design, express, and purify a range of human- and pathogen-derived proteins, leading to basic science discoveries that inform the development of vaccines and therapeutics. These discoveries have paved the way for candidate vaccines and therapies currently being manufactured under GMP standards for early-phase clinical trials. The Yeh Lab at DHVI specializes in investigating germinal center dynamics and antibody-forming responses following immunization. Their research provides critical insights that guide the design and selection of candidate vaccine immunogens.

 

Responsibilities

Flow Cytometry and Single B Cell Isolation:

Isolate and enrich B cells from human, macaque, and mouse samples.
Perform single B cell isolation and culture using flow cytometry techniques.
Design, execute, and analyze flow cytometry data using FlowJo and Prism GraphPad software.

Immunoassays

Conduct enzyme-linked immunosorbent assays (ELISA) and bead-based multiplex immunoassays.
Perform immunofluorescence staining and microscopic analyses to support experimental objectives.

Antibody Repertoire Studies

Isolate antibody genes from single-sorted or bulk-cultured B cells through PCR techniques.
Prepare samples for next-generation sequencing (NGS) library preparation.
Analyze NGS data to characterize antibody repertoires.
Manage recombinant antibody production, purification, and quality control.

Laboratory Safety And Compliance

Ensure adherence to all safety standards and procedures to maintain a safe laboratory environment.
Follow standard operating procedures (SOPs) and protocols with precision and consistency.
Maintain accurate, organized, and detailed records of experimental procedures and outcomes.

Laboratory Maintenance And Supply Management

Collaborate with the lab manager to ensure proper maintenance of equipment, reagents, and supplies.
Monitor project progress and coordinate the procurement of materials and reagents across multiple grants.

Skill Development And Collaboration

Develop and refine technical skills in collaboration with colleagues.
Participate in weekly reviews of primary data with the supervisor.
Collaborate with team members to support shared research goals and perform additional duties as directed by the Principal Investigator or senior staff.

Skills

Cell line and primary cell culture experience.
ELISA or bead-based immunoassays.
Nucleic acid extraction, RT-PCR, PCR, Real-time qPCR, and agarose gel electrophoresis.
Cell isolation using density gradient centrifugation, FACS and/or MACS-based methods.
Flow cytometer operation, surface/intracellular marker staining and flow data analysis with FlowJo software (plus but not required)
Mouse handling, tracking, sexing, anesthesia, blood withdraw, injection (tail vein intravenous, intraperitoneal, footpad and subcutaneous), and dissection. (plus but not required).
Must be team oriented and able to work in a highly collaborative and interactive work environment to complete complex tasks.
Must be able to manage multiple and rapidly changing priorities and have the ability to quickly learn new skills.
Must be detail-oriented and well organized.

ANTICIPATED PAY RANGE

Duke University provides an annual base salary range for this position as USD $ 50,439.00 to USD $76,417.00. Duke University considers factors such as (but not limited to) scope and responsibilities of the position; candidate' s work experience, education/training, and key skills; internal peer equity; as well as market and organizational considerations when extending an offer.

Your total compensation goes beyond the dollars on y our pay check. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array of family-friendly and cultural programs to eligible team members. Learn more a t:https://hr.duke.edu/benefits/

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/ or mental abilities. Additional information and provision for requests fo r reasonable accommodation will be provided by each hiring department.

Education/Training

REQUIRED QUALIFICATIONS AT THIS LEVEL

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

The Duke Human Vaccine Institute

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Lab Research Analyst I